(2pts) During the Purification of Lactate Dehydrogenase (LDH) experiment, you will need 50ml of buffer A150. Buffer A150 is 30mM Tris (pH 8.8) and 150mM NaCl. Given 1L of 1.5M Tris and 500ml of 5M NaCl, how much of each stock do you need to make 250ml of the A150 buffer

Answers

Answer 1

Answer:

The answer is "[tex]20 \ mL[/tex]"

Explanation:

Given:

Molarity= number of moles

because it is 1 Liter

[tex]\to \frac{0.03\ moles}{1.5 moles}=0.02\ L= 20 \ mL \ of\ Tris\\\\[/tex]

therefore,

it takes 20 mL of Tris.

[tex]\to \frac{0.150 \ moles}{5\ moles} =0.03\ L\\\\[/tex]

                     [tex]= 30 \ mL \ of\ Nacl[/tex]

So, take [tex]20 \ mL\ of\ NaCl.[/tex]


Related Questions

STRONGEST METALL ON EARTH

Answers

Answer:

tungstun

Explanation:

Answer:

tungsten

Explanation:

strongest out of any natural metal (142,000 psi).

When does a redox reaction produce electricity?
A. Only when an electrical device is attached
B. Whenever electrons are transferred between atoms
C. When electrons are transfered, and a pathway for electrons is provided
D. Whenever a pathway for electrons is provided

Answers

c. when electrons are transfered, and a pathway for electrons is provided.

help i’ll give u points

Answers

Answer:

60ug

Explanation:

If it has experienced two half lives, that means it has been halved twice. in that case, to undo it, just multiply it by two twice. 0.15ug * 2 = 0.30ug. 0.30ug * 2 = 0.60ug. Hope this helps.

A monoprotic weak acid when dissolved in water is 0.66% dissociated and produces a solution with a pH of 3.04. Calculate the Ka of the acid. g

Answers

Answer:

Ka = 6.02x10⁻⁶

Explanation:

The equilibrium that takes place is:

HA ⇄ H⁺ + A⁻Ka = [H⁺][A⁻]/[HA]

We calculate [H⁺] from the pH:

pH = -log[H⁺][H⁺] = [tex]10^{-pH}[/tex][H⁺] = 9.12x10⁻⁴ M

Keep in mind that [H⁺]=[A⁻].

As for [HA], we know the acid is 0.66% dissociated, in other words:

[HA] * 0.66/100 = [H⁺]

We calculate [HA]:

[HA] = 0.138 M

Finally we calculate the Ka:

Ka = [tex]\frac{[9.12x10^{-4}]*[9.12x10^{-4}]}{[0.138]}[/tex] = 6.02x10⁻⁶

B. Directions: Identify the factors affecting the different seasons in the Philippines.
1. Areas with less rainfall, has a pronounced dry
season. When it always rain, that place has wet season most of the year.
2. The higher the particular place, the cooler its
temperature.
3. Climate near in coastal areas is cooler because
water does not absorb heat as fast as land area.
4. . When large amount of warm air cool, if gives off
lots of rain over the land
5. Philippines lies near the equator, has warm climate
during certain times of the year.​

Answers

Answer:

The correct answer is -

1. Latitude

2. Altitude

3. Bodies of water

4. Wind system

5. Amount of rainfall

Explanation:

Lattitude is the angle from the earth's equator towards the north or south to the poles. This is one of the important factors that lead to different seasons and define the temperature of the area.

Altitude is the elevation from the sea that also helps in affecting the seasons as the higher the altitude cooler the temperature. Water bodies like the sea affect the wind and climate as they are not able to absorb heat faster in comparison to the land.

Wind also affects the seasons and climate of the area as a large amount of warm air cools, it gives off  lots of rain over the land. Precipitation or rainfall determines and influences the climate.

How does the neutron number in each compare

Answers

Answer:

Neutrons are all identical to each other, just as protons are. Atoms of a particular element must have the same number of protons but can have different numbers of neutrons.

Explanation:

Since the vast majority of an atom's mass is found its protons and neutrons, subtracting the number of protons (i.e. the atomic number) from the atomic mass will give you the calculated number of neutrons in the atom. In our example, this is: 14 (atomic mass) – 6 (number of protons) = 8 (number of neutrons).

g An ionic bond involves ____ a. molecules that gain or lose an atom to develop a bonding charge b. molecules that bond together through a weak force c. atoms that either lose or gain an electron in order to bond d. atoms that share an electron to tightly bond

Answers

Answer:

a. molecules that gain or lose an atom to develop a bonding charge

Explanation:

PLS HELP THESE ARE RHE ONLY QUESTIONS I HAVE LEFT DUE SOON

Answers

Explanation:

Magnesium reacts with dilute hydrochloric acid in a conical flask which is connected to an inverted measuring cylinder in a trough of water. The volume of hydrogen gas produced is measured over a few minutes, and the results are used to plot a graph

This is intended as a class practical. It is best if the students work in pairs because setting up and starting the experiment requires more than one pair of hands. One student can add the magnesium ribbon to the acid and stopper the flask, while the other starts the stopclock. During the experiment, one student can take the readings while the other records them. The experiment itself takes only a few minutes. But allow at least 30 minutes to give students time to set up, take readings and draw graph.

please mark as brainliest

1. An ion has a net charge of - 3, and 12 electrons, what is its atomic number? What element has that atomic number?

2. An ion of Celsius has a net charge of -5, how many electrons does it have?

3. Some atom has an atomic number of 46, how many electrons does it have if it has a net charge of +2?

4. How many neutrons are in an atom of Helium, and what is its mass number?

5. There are 3 neutrons in an isotope of Helium, what is its mass number?

6. n isotope has 83 neutrons and a mass number of 167, what is its atomic number? What element is defined by that atomic number?

7. What element has 54 neutrons and a mass number of 112?

8. What element has 10 electrons, a net charge of -2?

Answers

Answer:

19 because it gained 3 electron so 12-3=9 beryllium

2

3 is 46-2=44

4 is 2

5 is 5

6 84 polonium

7

8 oxygen

Explanation:

plz let me know if they are right or wrong:)

A solution has a pH of 2.5. what is the H+

Answers

Answer: The concentration of [tex]H^+[/tex] in the solution is [tex]3.16\times 10^{-3}M[/tex]

Explanation:

pH is defined as the negative logarithm of hydrogen ion concentration present in the solution.

[tex]pH=-\log [H^+][/tex]      .....(1)

We are given:

pH of solution = 2.5

Putting values in equation 1, we get:

[tex]2.5=-\log [H^+][/tex]

[tex][H^+]=10^{-2.5}[/tex]

[tex][H^+]=3.16\times 10^{-3}M[/tex]

Hence, the concentration of [tex]H^+[/tex] in the solution is [tex]3.16\times 10^{-3}M[/tex]

When aqueous solutions of AgNO3 and MgCl2 are mixed, a precipitate forms. What is the correct formula for the precipitate

Answers

Answer:

AgCl

Explanation:

The reaction that takes place is:

2AgNO₃ (aq) + MgCl₂ (aq) → Mg(NO₃) (aq) + 2AgCl (s)

The precipitate (meaning a solid substance) formed is silver chloride, AgCl.

All salts formed with silver and a halogen (F, Cl, Br, I) are insoluble in water, meaning that when working with aqueous solutions they will be precipitates.

Which additional product balances the reaction h2so4+2naoh>na2so4

Answers

Answer:

H 4so8 is the answer of balance the reaction

what will happen to the initial rate of the reaction if the concentration of iron(III) chloride is tripled

Answers

Answer:

Concentration and reaction rate is directly proportional

Explanation:

Basically, concentration is directly proportional to the concentration, so the higher the concentration, the higher the reaction rate.

Once the concentration is increased, more chemical will be added hence the reaction rate will increase

Which of the following is most likely to make a -3 ion?

P
Se
Be
All

Answers

The element whose atom may likely make a -3 ion is P.

Elements in the periodic table are classified into groups and periods. The elements in the same group have similar physical and chemical properties.

Phosphorus (P) is a member of group 15 in the periodic table. The most common ion formed by the members of this group is the -3 ion.

It is formed by accepting three electrons to complete their octet since they have five electrons on their outermost shell.

Hence P is most likely to form a -3 ion.

Learn more: https://brainly.com/question/11155928

What is the pH of a 0.086 M HCl solution?

Answers

Answer: pH = 1.066

Explanation: HCl is strong acid and it protolyzes totally:

HCl + H2O  ⇒ H3O+ + Cl-

Concentrations are same:  [H3O+] = c(HCl)

pH = - log[H3O+] = - log(0.086) = 1.0655

Calculate the number of cations and anions in each of the following compounds. Enter your answers in scientific notation. (a) 6.42 g of KBr:

Answers

Answer: The number of cations are [tex]3.24 \times 10^{22}[/tex] and number of anions are  [tex]3.24 \times 10^{22}[/tex] in 6.42 g of KBr.

Explanation:

The molar mass of KBr is (39.10 + 79.90) g/mol = 119.00 g/mol

Now, the dissociation equation for KBr is as follows.

[tex]KBr \rightarrow K^{+} + Br^{-}[/tex]

This means that 1 mole of KBr is forming 1 mole of [tex]K^{+}[/tex] (cation) and 1 mole of [tex]Br^{-}[/tex] (anion).

According to mole concept, 1 mole of every substance contains [tex]6.022 \times 10^{23}[/tex] atoms. Hence, number of cations present in 6.42 g KBr is calculated as follows.

[tex]No. of cations = Moles \times 6.022 \times 10^{23}\\= \frac{mass}{molar mass} \times 6.022 \times 10^{23}\\= \frac{6.42 g}{119.00 g/mol} \times 6.022 \times 10^{23}\\= 3.24 \times 10^{22}[/tex]

As according to the equation, there are equal number of moles of both cation and anions.

This means that the number of anions are also [tex]3.24 \times 10^{22}[/tex].

Thus, we can conclude that the number of cations are [tex]3.24 \times 10^{22}[/tex] and number of anions are  [tex]3.24 \times 10^{22}[/tex] in 6.42 g of KBr.

The number of cations and anions present in potassium bromide is 3.24 × 10²².

How we calculate atoms from moles?

In one mole of any substance 6.022 × 10²³ atoms of that substance is present and this is known as Avogadro's number.

KBr is a strong electrolyte means it fully dissociates into their constitute ions. So, the number of moles of produced ions is equal to the moles of KBr and dissociation is represented as:

KBr → K⁺ + Br⁻

From this it is clear that 1 mole of cation and 1 mole of anion is produced from 1 mole of KBr. Moles of KBr will be calculated as:

n = W/M, where

W = given mass = 6.42g

M = molar mass = 119

n = 6.42 / 119 = 0.053 moles

No. of cations and anions present in 0.053 moles = 0.053 × 6.022 × 10²³ = 3.24 × 10²².

Hence, 3.24 × 10²² is the no. of cations and anions.

To know more about Avogadro's number,  visit the below link:
https://brainly.com/question/10614569

Show the complete ionic equation and net ionic equation for all the equations below, then state whether or not a precipitate (insoluble compound) will form. To receive full credit, you must show ALL your work.
Cacl2(aq) + K2co3(aq) + -------->
Bacl2(aq) + MgSO4(aq) + -------->
AgNO3(aq) + Kl(aq) →
Nacl(aq) + (NH4)2Cro4(aq) →

Answers

Answer:

(a): Precipitate of calcium carbonate will form.

(b): Precipitate of barium sulfate will form.

(c): Precipitate of silver iodide will form.

(d): Precipitate of sodium chromate will form.

Explanation:

Complete ionic equation is defined as the equation in which all the substances that are strong electrolytes present in an aqueous state and are represented in the form of ions.

Net ionic equation is defined as the equations in which spectator ions are not included.

Spectator ions are the ones that are present equally on the reactant and product sides. They do not participate in the reaction.

(a):

The balanced molecular equation is:

[tex]CaCl_2(aq)+K_2CO_3(aq)\rightarrow 2KCl(aq)+CaCO_3(s)[/tex]

The complete ionic equation follows:

[tex]Ca^{2+}(aq)+2Cl^-(aq)+2K^+(aq)+CO_3^{2-}(aq)\rightarrow 2K^+(aq)+2Cl^-(aq)+CaCO_3(s)[/tex]

As potassium and chloride ions are present on both sides of the reaction. Thus, they are considered spectator ions.

The net ionic equation follows:

[tex]Ca^{2+}(aq)+CO_3^{2-}(aq)\rightarrow CaCO_3(s)[/tex]

Precipitate of calcium carbonate will form.

(b)

The balanced molecular equation is:

[tex]BaCl_2(aq)+MgSO_4(aq)\rightarrow MgCl_2(aq)+BaSO_4(s)[/tex]

The complete ionic equation follows:

[tex]Ba^{2+}(aq)+2Cl^-(aq)+Mg^{2+}(aq)+SO_4^{2-}(aq)\rightarrow Mg^{2+}(aq)+2Cl^-(aq)+BaSO_4(s)[/tex]

As magnesium and chloride ions are present on both sides of the reaction. Thus, they are considered spectator ions.

The net ionic equation follows:

[tex]Ba^{2+}(aq)+SO_4^{2-}(aq)\rightarrow BaSO_4(s)[/tex]

Precipitate of barium sulfate will form.

(c):

The balanced molecular equation is:

[tex]AgNO_3(aq)+KI(aq)\rightarrow KNO_3(aq)+AgI(s)[/tex]

The complete ionic equation follows:

[tex]Ag^{+}(aq)+NO_3^-(aq)+K^+(aq)+I^{-}(aq)\rightarrow K^+(aq)+NO_3^-(aq)+AgI(s)[/tex]

As potassium and nitrate ions are present on both sides of the reaction. Thus, they are considered spectator ions.

The net ionic equation follows:

[tex]Ag^{+}(aq)+I^{-}(aq)\rightarrow AgI(s)[/tex]

Precipitate of silver iodide will form.

(d):

The balanced molecular equation is:

[tex]2NaCl(aq)+(NH_4)_2CrO_4(aq)\rightarrow 2NH_4Cl(aq)+Na_2CrO_4(s)[/tex]

The complete ionic equation follows:

[tex]2Na^{+}(aq)+2Cl^-(aq)+2NH_4^+(aq)+CrO_4^{2-}(aq)\rightarrow 2NH_4^+(aq)+2Cl^-(aq)+Na_2CrO_4(s)[/tex]

As ammonium and chloride ions are present on both sides of the reaction. Thus, they are considered spectator ions.

The net ionic equation follows:

[tex]2Na^{+}(aq)+CrO_4^{2-}(aq)\rightarrow Na_2CrO_4(s)[/tex]

Precipitate of sodium chromate will form.

What is the law
of universal gravitation?

Answers

Answer:

[tex]{ \boxed{ \bf{newtons \: law \: of \: gravitation}}} [/tex]

It states that the product of masses of bodies ( planets and satellites and asteroids ) in space is inversely proportional to square of their separation mean distance

[tex]{ \tt{formular : F = \frac{GMm}{ {r}^{2} } }}[/tex]

Please help me I need help with this questions I’m very confused fused as to what the answer is please

Answers

Answer: This was because the experiment showed that a substance could emit radiation even while it was not exposed to light.

A student performs an experiment three times. The results are 4.52 g/mL, 4.54 g/mL, and 4.39 g/mL. Which of the following best describes these results with only the information given in this question?

a. high accuracy
b. high accuracy and high precision
c. high accuracy and low precision
d. high precision

Answers

I’m not sure but I believe the answer is D high precision

The pressure exerted by a gas container depends on

Answers

it’s due to the average number of collisions of gas molecules with the container walls per unit time. As such, pressure depends on the amount of gas (in number of molecules), its temperature, and the volume of the container.

HELP PLEASE!!!!
Can someone answer it as a paragraph please I should submit it today

Answers

Answer:

Jodi is violating two rules; She is not wearing any safety equipment and managed to catch fire to herself. She is also not wearing gloves or safety goggles like the other two. Kimberly is leaving fire unattended, and can cause many accidents and if chemicals come in contact in the wrong way, it could be catastrophic. Kimberly is also carelessly using a fire extinguisher carelessly and chemicals in the can cause accidents.

Explanation:

I hope this helps <3

This portion of the stratosphere if known as the _______.
A.
mesosphere
B.
tropopause
C.
thermosphere
D.
ozone layer

Answers

The answer to this question is B- tropopause

A researcher accidentally spilled some sand into his beaker containing crystalline compound X. Knowing what he did about melting points, he was not worried about obtaining the melting point of compound X with a few grains of sand present. Why

Answers

Answer:

See explanation

Explanation:

If we look at the structure of X, we will discover that X is an organic compound. The compound will certainly have a much lower melting point than sand.

This is because, sand is composed of high melting point inorganic materials.

Hence, even though the researcher accidentally spilled some sand into the the beaker containing the crystalline substance X, he does not need to be perturbed since the melting point of X is much lower than that of sand

What what I put to answer this?

Answers

The correct answer is B

What is the PH of a solution with a concentration of 5.2 x 10-8 M H3O?

Answers

Answer:

7.28

Explanation:

The pH of a solution can be found by using the formula

[tex]pH = - log [ { H_3O}^{+}][/tex]

From the question we have

[tex]ph = - log(5.2 \times {10}^{ - 8} ) \\ = 7.28399[/tex]

We have the final answer as

7.28

Hope this helps you

How much energy would it take to freeze 12.5 g of water?

Answers

30 minutes to be exact

The amount of energy needed to freeze 12.5 grams of water into ice is equals to 14.295 kilo joules.

How do we calculate energy?

Energy required to freeze water will be calculated by using the following equation as:

Q = mcT, where

m = mass of water = 12.5g = 0.0125kg

c = specific heat of water = 4.187 kJ/kg.K

T = temperature to freeze = 0 degree C = 273.15 K

On putting values we get,

Q = (0.0125)(4.187)(273.15)

Q = 14.295 kJ

Hence required amount of energy is 14.295 kJ.

To know more about absorbed energy, visit the below link:

https://brainly.com/question/26306578

A 0.2264 g sample of a pure carbonate, XnCO3(s) , was dissolved in 50.0 mL of 0.1800 M HCl(aq) . The excess HCl(aq) was back titrated with 24.90 mL of 0.0980 M NaOH(aq) . How many moles of HCl react with the carbonate

Answers

Answer:

6.56x10⁻³ mol

Explanation:

First we calculate how many HCl moles are there in 50.0 mL of a 0.1800 M solution:

0.1800 M * 50.0 mL = 9.00 mmol HCl

Then we need to calculate how many HCl moles were in excess, that number is the same as the number of NaOH moles they reacted with:

0.0980 M * 24.90 mL = 2.44 mmol NaOH = 2.44 mmol HCl

Finally we calculate the difference between the original number of HCl moles and the number remaining after the reaction with XnCO₃:

9.00 mmol - 2.44 mmol = 6.56 mmol6.56 mmol / 1000 = 6.56x10⁻³ mol

Draw the structure of the major organic product isolated from the reaction of 3-hexyne with hydrogen (1 mol), Lindlar palladium.

Answers

Answer:

See explanation and image attached

Explanation:

When 1 mole of hydrogen is added to 3-hexyne, the addition occurs on the same face or side of the triple bond to yield cis-2-hexene in the presence of lindlar catalyst which prevents further hydrogenation. This is known as syn addition.

The syn addition is achieved because the hydrogen molecule is first adsorbed on the surface of the palladium metal and is subsequently attached to the same face of the triple bond in 3-hexyne as shown in the image attached to this answer.

If a light beam strikes a shiny object at a 90-degree angle, to what are the angle of incidence and the angle of reflection equal?

Answers

Answer:

Angle of incidence = angle of reflection = 0

Explanation:

Given that,

A light beam strikes a shiny object at a 90-degree angle.

The angle made by incident ray and normal to the mirror is called the angle of incidence and the angle made by the reflected ray and the normal is called the angle of reflection.

Here, the beam strikes normally. So, the angle of incidence is 0.

A/c to the law of reflection,

Angle of incidence = angle of reflection

So, the angle of reflection is also equal to 0.

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